Isolation and Identification of Catechin by a New Method from Food Efficiency Stimulating Plant Alhagi camelorum

Abdul Hafeez Laghari; Shahabuddin Memon; Aisha Nelofar; Khalid Mohammad. Khan

doi:10.52763/PJSIR.PHYS.SCI.60.2.2017.59.66

Abdul Hafeez Laghari PCSIR Laboratories Complex, Karachi-75280, Pakistan
Shahabuddin Memon National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan
Aisha Nelofar PCSIR Laboratories Complex, Karachi-75280, Pakistan
Khalid Mohammad. Khan H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

DOI: https://doi.org/10.52763/PJSIR.PHYS.SCI.60.2.2017.59.66

Keywords: catechin, Alhagi camelorum, HPLC, isolation

Abstract

The present article describes a new developed method for the simultaneous determination of
(+)-catechin (1) and (-)-epicatechin (2), separated via HPLC. The method has been validated and applied
on the real samples and 1 has been detected in both aerial and root parts of Alhagi camelorum without any
ambiguity of fake positive or negative presence of 1 or 2 by virtue of dual detection system of UV and
Mass Spectrometry. The antioxidant capacity was also investigated and a linear correlation has been noticed
between the antioxidant capacity and the catechin amount in A. camelorum extracts

Isolation and Identification of Catechin by a New Method from Food Efficiency Stimulating Plant Alhagi camelorum

Catechin Isolation from Alhagi camelorum

Abstract